NMR spectroscopy
NMR Spectroscopy
- High-resolution NMR spectroscopy for biological fluids and tissue samples
- High-resolution magic angle spinning (HR-MAS) MR spectroscopy
- Automation of NMR spectroscopy using Sample Jet
Ernst
- 600 MHz Bruker Avance Neo NMR spectrometer dedicated for biofluids
- Automation with Sample Jet
- Probes: 5 mm BBI, 5 mm BBO, 10 mm BBO
Andrew
- 600 MHz Bruker Avance III NMR spectrometer dedicated for tissue samples.
- Magic angle spinning (MAS) probeheads
- Dual probehead: 1H / 13C
- Triple probehead: 1H / [31P, 13C]
Pre-Clinical Screening and In Vitro Diagnostics research (IVDr)
One of our 600 MHz NMR instruments is dedicated to metabolomics and liquid samples. This instrument is compatible with Bruker Biospins IVDr platform, allowing high performing and cost-effective analysis for pre-clinical screening and in vitro diagnostics research.
- For serum/plasma samples we provide fully automated quantification of 41 metabolites and lipoprotein subclass analysis.
- For urine samples we provide fully automated quantification of up to 150 metabolites.
We also perform analysis of other biofluids, but presently without automated quantification.
Please contact us if you have research proposals involving other biofluids and we will see how we may help you.
The required sample volume may vary between 120 – 560 μL, depending on tube size and biofluid. For optimal results we recommend 320 μL for serum/plasma and 560 μL for urine. NMR spectroscopy is inherently non-destructive, meaning that the samples may be returned after analysis.
Highlighted publications
NMR analysis of biofluids
- Wang F, Debik J, Andreassen T, Euceda LR, Haukaas TH, Cannet C, Schäfer H,Bathen TF, Giskeødegård GF. Effect of Repeated Freeze-Thaw Cycles on NMR-Measured Lipoproteins and Metabolites in Biofluids. J Proteome Res. 2019 Oct 4;18(10):3681-3688. doi: 10.1021/acs.jproteome.9b00343. Epub 2019 Sep 11. PMID: 31476120.
- Debik J, Euceda LR, Lundgren S, Gythfeldt HVL, Garred Ø, Borgen E, Engebraaten O, Bathen TF, Giskeødegård GF. Assessing Treatment Response and Prognosis by Serum and Tissue Metabolomics in Breast Cancer Patients. J Proteome Res. 2019 Oct 4;18(10):3649-3660. doi: 10.1021/acs.jproteome.9b00316. Epub 2019 Sep 16. PMID: 31483662.
- Giskeødegård GF, Andreassen T, Bertilsson H, Tessem MB, Bathen TF. The effect of sampling procedures and day-to-day variations in metabolomics studies of biofluids. Anal Chim Acta. 2019 Nov 12;1081:93-102. doi:10.1016/j.aca.2019.07.026. Epub 2019 Jul 17. PMID:31446969.
- Madssen TS, Thune I, Flote VG, Lundgren S, Bertheussen GF, Frydenberg H, Wist E, Schlichting E, Schäfer H, Fjøsne HE, Vettukattil R, Lømo J, Bathen TF,Giskeødegård GF. Metabolite and lipoprotein responses and prediction of weight gain during breast cancer treatment. Br J Cancer. 2018 Oct;119(9):1144-1154. doi: 10.1038/s41416-018-0211-x. Epub 2018 Nov 7. PMID: 30401977; PMCID: PMC6220113.
NMR analysis tissue
- Haukaas TH, Euceda LR, Giskeødegård GF, Bathen TF. Metabolic Portraits of Breast Cancer by HR MAS MR Spectroscopy of Intact Tissue Samples. Metabolites. 2017 May 16;7(2):18. doi: 10.3390/metabo7020018. PMID: 28509845; PMCID:PMC5487989.
- Haukaas TH, Euceda LR, Giskeødegård GF, Lamichhane S, Krohn M, Jernström S, Aure MR, Lingjærde OC, Schlichting E, Garred Ø, Due EU, Mills GB, Sahlberg KK,Børresen-Dale AL, Bathen TF; Oslo Breast Cancer Consortium (OSBREAC). Metabolic clusters of breast cancer in relation to gene- and protein expression subtypes. Cancer Metab. 2016 Jun 27;4:12. doi: 10.1186/s40170-016-0152-x. PMID: 27350877; PMCID: PMC4922058.
Contact
Contact
Visiting address
MR Core Facility
Olav Kyrres gt 11, Trondheim
Postal address
MR Core Facility
MTFS
Norwegian University of Science and Technology
Post box 8905
N-7491 Trondheim
Norway