Mass Spectrometry Imaging (MSI)

MSI is based on acquiring mass spectra pixel by pixel on tissue sections, which allows the spatial distributions of hundreds of different masses of for example metabolites or phospholipids to be captured at a time. All types of tissue sections and cell culture samples can be measured, from human heterogeneous tumor samples to plant tissue to organoid cultures. The rapifleX instrument at our facility has a supported maximal spatial resolution of 20 µm, but we could successfully image at even higher resolutions of 10 µm and 5 µm. This technique is nondestructive for most of the tissue section, meaning that the remaining tissue can be subjected to a broad variety of histology staining afterwards, allowing for a direct comparison between MSI and histology.

Special application MSI: Multiplexed MALDI-IHC

Multiplexed MALDI-IHC is the combination of immune histochemistry (IHC) with MALDI MSI for detection of mass-tag labeled anti-bodies. This technology allows extreme high-plexity by simultaneous staining and detection of hundreds of targets on the same tissue section. Multiplexed MALDI-IHC can be combined with conventional untargeted MSI (for example for metabolite and phospholipid imaging) and conventional microscopy-based IHC on the same tissue section for maximal utilization. We have required equipment and established protocols to perform the Miralys highplex assay from AmberGen readily available at MR Core Facility.

Peptide Mass Fingerprinting and other MS-based Proteomics Applications

MR Core Facility provides a joint service together with the Proteomics and Modomics Experimental Core Facility (PROMEC) for conventional MALDI-MS-based proteomics where liquid-chromatography is not required. Sample preparation can be performed at PROMEC while the MS analysis can be performed on our MALDI-TOF instrument. Please contact us for more information.

• Giampà, M., Andersen, M. K., Krossa, S., Denti, V., Smith, A., Moestue, S. A. (2023). Visualization of Small Intact Proteins in Breast Cancer FFPE Tissue. Methods Mol Biol. 2688:161-172. doi:10.1007/978-1-0716-3319-9_14
• Andersen, M. K., Giampà, M., Midtbust, E., Høiem, T.S., Krossa, S., Tessem, M.B. (2023). Sample Preparation for Metabolite Detection in Mass Spectrometry Imaging. Methods Mol Biol. 2688:135-146. doi:10.1007/978-1-0716-3319-9_12
• Krossa, S., Andersen, M. K., Midtbust, E., Viset, T., Størkersen, Ø., Giskeødegård, G. F., Rye, M. B., Tessem, M.-B. (2022). Inflammatory chemokines upregulated in the tumor microenvironment of prostate cancer patients experiencing relapse after radical prostatectomy. European Urology 81 (S1389).
• Andersen, M. K., Krossa, S., Midtbust, E., Viset, T., Størkersen, Ø., Giskeødegård, G. F., Rye, M. B., Tessem, M.-B. (2022). Multiomics spatial mapping of lipid metabolism in prostate cancer. European Urology 81 (S1386 - S1387).
• Buchholz, R., Krossa, S., Andersen, M. K., Holtkamp, M., Sperling, M., Karst, U., Tessem, M.-B. (2022). A simple preparation protocol for shipping and storage of tissue sections for laser ablation-inductively coupled plasma-mass spectrometry imaging. Metallomics 14 (3). 
• Høiem, T. S., Andersen, M. K., Martin-Lorenzo, M., Longuespée, R., Claes, B. S. R., Nordborg, A., Dewez, F., Balluff, B., Giampà, M., Sharma, A., Hagen, L., Heeren, R. M. A., Bathen, T. F., Giskeødegård, G. F., Krossa, S., Tessem, M.-B. (2022). An optimized MALDI MSI protocol for spatial detection of tryptic peptides in fresh frozen prostate tissue. Proteomics. Feb 16:e2100223. doi: 10.1002/pmic.202100223. Epub ahead of print.
• Andersen, M. K., Høiem, T. S., Claes, B. S. R., Balluff, B., Martin-Lorenzo, M., Richardsen, E., Krossa, S., Bertilsson, H., Heeren, R. M. A., Rye, M. B., Giskeødegård, G. F., Bathen, T. F., Tessem, M.-B. (2021). Spatial differentiation of metabolism in prostate cancer tissue by MALDI-TOF MSI. Cancer Metab. 9(1): 9.
• Andersen, M. K., Krossa, S., Høiem, T. S., Buchholz, R., Claes, B. S.R., Balluff, B., Ellis, S., Richardsen, E., Bertilsson, H., Heeren, R. M.A., Bathen, T. F., Karst, U., Giskeødegård, G. F., Tessem, M.-B. (2020). Simultaneous Detection of Zinc and Its Pathway Metabolites Using MALDI MS Imaging of Prostate Tissue. Analytical Chemistry. vol. 92 (4)
• Andersen, M. K., Krossa, S., Høiem, T. S., Buchholz, R., Claes, B. S.R., Balluff, B., Ellis, S., Richardsen, E., Bertilsson, H., Heeren, R. M.A., Bathen, T. F., Karst, U., Giskeødegård, G. F., Tessem, M.-B. (2019). Simultaneous detection of the cancer biomarkers zinc and citrate in prostate cancer tissue using mass spectrometry imaging. European urology. Supplement. vol. 18 (8).