We are a lab within the Biopolymer and Biomaterials research group at the Department of Biotechnology and Food Science.  Our main research interest is the study of the structures and mechanisms of carbohydrate-active enzymes (CAZymes). In particular, focusing on  protein dynamics and interactions between proteins and carbohydrates.

Our approach to research

In our research group, we recognize that no single technique provides a complete understanding of complex systems, such as enzymes acting on polysaccharides. To address this, we combine various biophysical techniques and computational models, enabling us to derive deeper insights than what any individual method could achieve.

How we build a unique research competence:

• Prioritize robust protein production, as obtaining sufficient protein is often a bottleneck in biophysical and biochemical investigations.
• Develop expertise in a wide range of biophysical and biochemical techniques including biomolecular NMR spectroscopy, surface-plasmon resonance (SPR), circular dichroism, and dynamic light scattering.
• Emphasize the integration of experimental data with computational models.
• Maintain a commitment to open science practices and methodological rigor.

Current projects

Protein-(bio)polymer interactions

Funding: Department of Biotechnology and Food Science, NTNU

• Characterization of enzymatic reactions on insoluble substrates (cellulose, chitin, PET).
• Time course analysis by NMR.
• Methodology for measuring kinetics and affinity of protein binding to insoluble substrates.
• SPR to study protein interactions with polysaccharide thin films

Xanthan glycosyltransferases

Funding: Novo Nordisk Foundation

• Molecular dynamics simulations of xanthan GTs.
• Computational modelling of substrate-enzyme interactions.
• Designing and engineering substrate specificities.
• Characterizing physical properties of xanthan variants.

Our approach to training

Training at the master’s level provides an invaluable opportunity for hands-on research experience. We focus on individualized training plans with specific learning goals to equip students with relevant skills. Students in our group gain exposure to a wide array of biophysical and characterization techniques, as well as computational modeling of biomolecules, and are always included as co-authors on papers resulting from their research. Contact us if you are interested in doing your master’s in our group. Available projects are listed below, but feel free to suggest your own project ideas. We have also hosted placement students through the ERASMUS+ programme.

Current master projects

• Biomaterial Breakthroughs: Streamlined polysaccharide engineering without cells
• Plastic-degrading enzymes
• Extracellular ATP: The secret language of plants?
• Exploring Substrate Binding Conformations in Xanthan Gum Glycosyltransferases: A Computational Comparative Study
• Computational Investigation of the Interaction between a Membrane-Anchored Oligosaccharide and an Integral Protein Domain
• Exploring the synergistic activity of cellulose- and plastic-degrading enzymes on composite cellulose/PET materials