Proteomics Services
Proteomics Analyses
Samples
We work with proteins or peptides in many states. They can be in solution, dried or in a gel band. We can process and analyze cell cultures, tissues, biological fluids and others (for example: painting shavings).
Wetlab Services
Purification of proteins and peptides.
Separation by (1D/2D)-PAGE, 1D/2D-western, capillary western analysis and offgel isoelectric focusing.
Preparation of samples for LC/MS analysis.
Enrichment of Post-translational Modifications.
LC/MS-based Analyses
Identification.
Quantification.
Post-translational Modifications, mainly: Phosphorylation and Ubiquitination.
Intact Protein.
Protein Interactions.
De-novo Sequencing.
Practical Information
Ideally, contact us at early stages regarding your proteomics analysis; if possible, before applying for funding so that we can help estimate the costs. so that they can ne included in your application. Also, several projects aim at identifying/quantifying tricky proteins (low abundance, PTMed, highly hydrophobic etc.), we can help with advice on sample preparation steps so that achieving your aim is possible.