Cellular & Molecular Imaging Core Facility (CMIC)

CMIC offers the combination of instruments and competence that you need to image and quantify biological processes. We provide services, training, and scientific consultation in advanced light microscopy and histology.

Our labs

CMIC is a collaboration between two different laboratories:

The Advanced Light Microscopy Lab at CMIC provides access to a range of imaging systems for superresolution, live-cell, and high-throughput imaging. The facility is equipped with STED superresolution, confocal, high-speed, and high-throughput fluorescence microscopes, as well as TIRF microscopy. Training and technical support are available for both basic and advanced microscopy techniques. The facility also offers image analysis services, with dedicated workstations and support for developing custom pipelines in Python.

The Histology Lab offers a full range of services from tissue processing to digital imaging. We provide tissue embedding, sectioning (cryo and paraffin), routine and special stainings, immunohistochemistry (IHC), and digital slide scanning in bright field, fluorescence, phase contrast, or polarized light. Researchers are encouraged to consult us early in their project planning to ensure optimal sample preparation and workflow.

Publications

Below is a list of recent publications in which CMIC has played a key role by providing essential instruments, technical assistance, or image analysis, helping make these publications possible. Please remember to cite our contribution using these guidelines.

Hayran, A. B. et al. RPA guides UNG to uracil in ssDNA to facilitate antibody class switching and repair of mutagenic uracil at the replication fork. Nucleic Acids Research 52, 784 (2024).

Díez-Sánchez, A. et al. LSD1 drives intestinal epithelial maturation and controls small intestinal immune cell composition independent of microbiota in a murine model. Nature Communications 2024 15:1 15, 1–20 (2024).

Sadeghinia, M. J. et al. Quantified planar collagen distribution in healthy and degenerative mitral valve: biomechanical and clinical implications. Scientific Reports 2024 14:1 14, 1–13 (2024).

Andersen, M. K. et al. Spatial transcriptomics reveals strong association between SFRP4 and extracellular matrix remodeling in prostate cancer. Nature Communications Biology 2024 7:1 7, 1–15 (2024).

D’Gama, P. P. et al. Ciliogenesis defects after neurulation impact brain development and neuronal activity in larval zebrafish. iScience 27, (2024).

Bordin, D. L. et al. Loss of alkyladenine DNA glycosylase alters gene expression in the developing mouse brain and leads to reduced anxiety and improved memory. DNA repair 135, (2024).

Mediaas, S. D. et al. Metformin improves Mycobacterium avium infection by strengthening macrophage antimicrobial functions. Frontiers in Immunology 15, 1463224 (2024).

Sandbakken, E. T. et al. Biofilm and the effect of sonication in a chronic Staphylococcus epidermidis orthopedic in vivo implant infection model. Journal of orthopaedic surgery and research 19, (2024).

Quinsgaard, E. M. B., Korsnes, M. S., Korsnes, R. & Moestue, S. A. Single-cell tracking as a tool for studying EMT-phenotypes. Experimental Cell Research 437, 113993 (2024).

Bugaj, A. M. et al. Dissecting gene expression networks in the developing hippocampus through the lens of NEIL3 depletion. Progress in Neurobiology 235, 102599 (2024).

Saastad, S. A., Skjervold, A. H., Ytterhus, B., Engstrøm, M. J. & Bofin, A. M. PD-L1 protein expression in breast cancer. Journal of Clinical Pathology 77, 730–736 (2024).

Mørk, E. et al. Clinical versus Histological Assessment of Basal Cell Carcinoma Subtype and Thickness of Tumours Selected for Photodynamic Therapy. Acta Dermato-Venereologica 104, 18308 (2024).

Christensen, E. et al. New, simplified versus standard photodynamic therapy (PDT) regimen for superficial and nodular basal cell carcinoma (BCC): A single-blind, non-inferiority, randomised controlled multicentre study. PLOS ONE 19, e0299718 (2024).

Vornewald, P. M. et al. Mmp17-deficient mice exhibit heightened goblet cell effector expression in the colon and increased resistance to chronic Trichuris muris infection. Frontiers in Immunology 14, 1243528 (2023).

Lin, X. et al. A loss-of-function mutation in human Oxidation Resistance 1 disrupts the spatial–temporal regulation of histone arginine methylation in neurodevelopment. Genome Biology 24, (2023).

Skjervold, A. H., Valla, M., Ytterhus, B. & Bofin, A. M. PAK1 copy number in breast cancer-Associations with proliferation and molecular subtypes. PloS one 18, (2023).

Skjervold, A. H., Valla, M. & Bofin, A. M. Oestrogen receptor low positive breast cancer: associations with prognosis. Breast cancer research and treatment 201, 535–545 (2023).

Bugaeva, O. et al. Tumour Suppressor Neuron Navigator 3 and Matrix Metalloproteinase 14 are Co-expressed in Most Melanomas but Downregulated in Thick Tumours. Acta dermato-venereologica 103, (2023).

Nilsen, K. E. et al. Peptide derived from SLAMF1 prevents TLR4-mediated inflammation in vitro and in vivo. Life science alliance 6, (2023).

Sulheim, E. et al. Contrast Enhanced Magnetic Resonance Imaging of Amyloid-β Plaques in a Murine Alzheimer’s Disease Model. Journal of Alzheimer’s Disease 93, 411 (2023).

Snipstad, S., Bremnes, F., Dehli Haugum, M. & Sulheim, E. Characterization of immune cell populations in syngeneic murine tumor models. Cancer Medicine 12, 11589–11601 (2023).

Mühlenpfordt, M. et al. Real-Time Intravital Imaging of Acoustic Cluster Therapy–Induced Vascular Effects in the Murine Brain. Ultrasound in Medicine & Biology 49, 1212–1226 (2023).

Haram, M. et al. Ultrasound and Microbubbles Increase the Uptake of Platinum in Murine Orthotopic Pancreatic Tumors. Ultrasound in Medicine & Biology 49, 1275–1287 (2023).

Gederaas, O. A. et al. Proteomic analysis reveals mechanisms underlying increased efficacy of bleomycin by photochemical internalization in bladder cancer cells. Molecular Omics 19, 585–597 (2023).

Bokil, A. A. et al. Discovery of a new marker to identify myeloid cells associated with metastatic breast tumours. Cancer Cell International 23, 1–18 (2023).

Børkja, M. L. B. et al. S100A8 gene copy number and protein expression in breast cancer: associations with proliferation, histopathological grade and molecular subtypes. Breast cancer research and treatment 201, 339–350 (2023).

Andersen, M. K. et al. Sample Preparation for Metabolite Detection in Mass Spectrometry Imaging. Methods in Molecular Biology 2688, 135–146 (2023).

Gopalakrishnan, S. et al. Comprehensive protocols for culturing and molecular biological analysis of IBD patient-derived colon epithelial organoids. Frontiers in Immunology 14, 1097383 (2023).

Walaas, G. A. et al. Physiological hypoxia improves growth and functional differentiation of human intestinal epithelial organoids. Frontiers in Immunology 14, 1095812 (2023).

Fernandez, J. L. et al. A Comparative Analysis of Orthotopic and Subcutaneous Pancreatic Tumour Models: Tumour Microenvironment and Drug Delivery. Cancers 15, 5415 (2023).

Mørk, E., Mjønes, P., Foss, O. A., Bachmann, I. M. & Christensen, E. Expression of β-Catenin, E-Cadherin, and α-Smooth Muscle Actin in Basal Cell Carcinoma Before Photodynamic Therapy in Non-recurrent and Recurrent Tumors: Exploring the Ability of Predicting Photodynamic Therapy Outcome. Journal of Histochemistry and Cytochemistry 71, 111 (2023).

Publications from CMIC and CMIC users 2012-2016 (pdf).