Course content

The course includes relevant laboratory techniques that are put together into comprehensive exercises. Methods such as end-point PCR and/ or real-time PCR. Furthermore, RNA isolation, cell culture, cDNA synthesis, qPCR, flow cytometry and protein analysis are combined to look at cellular stress. The course concludes with a process and analysis where, among other things, analysis of lipids / proteins / carbohydrates gives a little insight into industrial biotechnology.

Learning outcome

Knowledge - the student has:

• knowledge of biotechnological methods applied to both prokaryotic and eukaryotic cells including; nucleic acids, proteins and organic lipids
• knowledge of combining biotechnological methods to achieve the desired result
• knowledge of various procedures for biotechnological analysis and their application

Skills - the student:

• can apply and utilize procedures for biotechnological methods
• can perform biotechnological analyzes in the laboratory
• masters key techniques for isolation, purification and characterization of biomolecules.
• has skills in using cell and molecular biological methods at advanced level
• can handle chemical substances and biological material in accordance to current HSE regulations, and understand the environmental consequences associated with the activity
• have the skills to assess and perform analyzes in a safe and sustainable way

Competence - the student has:

• competence in biotechnological analysis methods and how these can be used in a safe and sustainable way
• expertise in modern biotechnological methods for the production and analysis of nucleic acids, proteins and organic lipids
• competence in assessing the ethical aspects by applying biotechnological methods
• competence in communicating both written and oral independent work and research including reading of scientific literature and use of sources
• competence to contribute to new thinking and innovation

Learning methods and activities

Compulsory laboratory exercises. Up to 15 lab days of 8 hours, with associated journal book must be approved before the student is allowed to sit for the exam. The journal book is approved after completion of the lab course. Estimated study time for the course is 400 hours, of which 150 are organized laboratory courses and lectures, and 100 hours are expected for preparation for the laboratory course

Compulsory assignments

• Mandatory assignment

Specific conditions

Required previous knowledge

BTA1001 General micorbiology, HBIOA1003 Biochemistry with cell biology, HBIOA1004 General chemistry and BTA2004 Basic biotechnology HBIOA2004 Cell- and molecular biology

Credit reductions