Bioprocess and Microbial Physiology
Bioprocess and Microbial Physiology
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The Bruheim lab focuses on Deep Phenotyping of prokaryotic and eukaryotic model systems using Advanced Cultivation Technology and Mass Spectrometry based Metabolomics, Fluxomics and Lipidomics. Bruheim is scientific leader of the NV faculty Mass Spectrometry lab. We have developed a suite of target and non-target MS methods, which we apply on a range of biological model systems and research questions.
Our model systems
E. coli
Antibiotic resistance development, stress responding, metabolic burden, tolerance and persistence, recombinant protein production
Streptomyces coelicolor
Antibiotic/ secondary metabolite production
Yeast
Beer, wine, basic studies
Zymomonas mobilis
Biofuel production
Thraustochytrids
Omega3 fatty acid production
Salmon
Lipid profiling, omega3 fatty acid metabolism
Human cell culture
Cancer and immune metabolism, hypoxia, “cultured meat”
We have also a number of external collaborations in Metabolomics, Fluxomics and Lipidomics that involves e g. screening of urine, serum, plasma samples and lipid profiling in mouse, goat, dog, cod, etc
Core Expertise
- Bioprocess and fermentation technology, Microbial Physiology
- MS based Metabolomics, Fluxomics and Lipidomics; focus on both method development and applications
Resources
- Microbial bioreactor lab (9 Eppendorf and Applikon bench-top fermenters, 0.1-2 L) for advanced cultivation studies (batch, fed-batch, continuous, mass spectrometer off-gas analysis)
- BioLectorPro microbioreactor system
- Cell culture lab including hypoxia chamber
- General microbial and biochemistry labs with all equipment needed for state-of-the-art analyses
Projects (2023)
- Trond Mohn Stiftelse: “Targeting AMR by inhibition of bacterial stress responses”
- RCN Center for Research based Innovation in Industrial Biotechnology
Sub-projects: “Development and application of fluxomic tools to develop microbial cell factories for industrial cultivation conditions” and “Optimized bulk starter cultures for cheese production” - NTNU Biotechnology programme: “Chemically defined cell culture media”
- NTNU IBT PhD: “Inhibition of antibiotic resistance development through targeting bacterial translesion synthesis mechanism with novel peptide drugs”
- NTNU IBT: “Genetic and molecular investigation of Norwegian Farmhouse Ale yeasts”
Members (2023)
-
Laura Garcia Calvo
+4792132755 laura.g.calvo@ntnu.no Department of Biotechnology and Food Science -
Kåre Andre Kristiansen Head Engineer
+47-73412377 +4798858932 kare.a.kristiansen@ntnu.no Department of Biotechnology and Food Science -
Divyata Vilas Rane Researcher
+4794806186 divyata.rane@ntnu.no Department of Biotechnology and Food Science -
Lilja Brekke Thorfinnsdottir Head Engineer
+4799396927 lilja.b.thorfinnsdottir@ntnu.no Department of Biomedical Laboratory Science
including 6-8 MSc students