Scientifica SliceScope MP Microscope - Center for Advanced Microscopy (CAM)
Scientifica SliceScope MP Microscope
The InVivo SliceScope is a multiphoton microscope located at the animal facility (The Comparative Medicine Core facility) at St. Olav’s Hospital.
The microscope is fully motorized and software controlled. A scan speed up to 30 frames per second (512 x 512 pixels) can be achieved. It is equipped with an infrared pulsed laser for multiphoton (MP) fluorescence and second harmonic signal generation (SHG), four sensitive GaAsp detectors and a camera for widefield fluorescence microscopy.
The microscope
The Scientifica InVivo SliceScope has a modular design and is fully motorized: XY base plate (50 mm travel in X and Y) with patch pad, nosepiece, IR pulsed laser operation and power control. The microscope is designed for animal experiments and reflected light observations. For epifluorescence observation, the system is equipped with an Olympus BX reflected illuminator with turret for 6 fluorescence cubes and a trinocular tube with eyepieces and a camera port.
Focus drives
- Motorized objective nosepiece (26 mm travel range in Z)
- Pifoc Z-focus module (400 um travel range in Z) for higher speed and accuracy.
Scanners
- Conventional galvo scanner: 4 frames/sec at 512 x 512 pixels image format
- Resonant scanner: 30 frames/sec at 512 x 512 pixels image format
Objective
XLUMPLFLN 20x, NA 1, WD 2 mm, water immersion. Other objectives can be used.
Laser
MaiTai DeepSee Ti:Sp system from SpectraPhysics. Tuning range is 690 to 1040 nm (80 MHz repetition rate). Max power at 800 nm is 3 W. Integrated and automated dispersion control. The laser is software-controlled.
Detectors
Four GaAsP detectors (Chromoflex unit) from Hamamatsu (H10771P-40) in reflection mode.
Filter sets for multiphoton and SHG in front of GaAsP
| Channel | Emission filter |
|---|---|
| 1 (SHG) | 390/18 nm |
| 2 (DAPI) | 460/60 nm |
| 3 (Nilred) | LP 590 nm |
| 4 (GFP) | 525/50 nm |
Light for epi-fluorescence
LEDs pE-300 from CoolLED with liquid light guide.
Filter cubes for epi-fluorescence
| Filter cube | Excitation filter | Emission filter | Dichroic |
|---|---|---|---|
| BF | For reflected brightfield | ||
| DAPI | 350/50 nm | 460/50 nm | 500 nm |
| GFP | 470/70 nm | 525/50 nm | 495 nm |
| TRITC | 535/50 nm | 610/75 nm | 565 nm |
| CY5 (Nilered) | 470/40 nm | LP 590 nm | 550 nm |
Camera
Andor Zyla sCMOS camera 5.5 MPix mono, 200 fps.
Software on the computer
- LinLab for patch pad and nosepiece control
- MaiTai software for operating the laser
- SciScan version 1.3 for microscope control and laser control (laser ON/OFF and pockel cell control for power adjustments)
- LabView and NI MAX for controlling the different hardware components
Accessories
Tiltable nosepice