Leica DM6 FS Fluorescence Microscope - Center for Advanced Microscopy (CAM)
Leica DM6 FS Fluorescence Microscope
– Upright brightfield and epifluorescence microscope
The microscope is a modular, motorized widefield system for brightfield, phase and epifluorescence microscopy.
The microscope
The Leica DM6 FS is an upright microscope with a detachable motorized stage (76 x 50 mm adjustment range), XYZ ergo control panel, a holder for a single objective, two camera ports and five filter cubes.
Focus drives
- Motorized objective nosepiece (travel range 13 mm, z-step size 10 nm)
Objectives
| Magnification | NA | Type | Immersion | Coverslip | WD (mm) |
|---|---|---|---|---|---|
| 4x | 0.13 Ph0 | HC PL Fluotar | Air | -/ 0.17 | ~12 |
| 20x | 0.5 | HCX APO L | Water | - (dipping) | 3.5 |
| 20x | 0.4 Ph1 | HCX PL Fluotar L | Air | 0-2 (Corr) | 6.9 |
Condenser lens
NA 0.90, air, working distance 1 mm, with phase rings
Light for epi-fluorescence and transmitted light
- External EL 6000 (HXP 120 W): fast shutter, fiber-optics and bulb lifetime 2000h
- Color LED for transmitted light.
Filter cubes for epifluorescences
- DAPI/Hoechst 33258
- AF 488/GFP/CY2
- AF 546/GFP/CY3
- AF 633/GFP/CY5
- NIR dyes/CY7
Cameras
- Leica DFC700 T color CCD camera, 2.8 MPix, 40 fps at max resolution
- Andor Zyla sCMOS camera 5.5 MPix mono, 200 fps.
Software on the computer
- Andor Solis (specific for the Andor camera)
- Leica LAS X with time-lapse, z-control, multi-channel acquisition, tile imaging and Navigator module for high content screening and automated microscopy.
The Andor camera can be controlled by LAS X.