This project was based on previous results from the group showing cell-cycle dependent regulation of genes epigenetically silenced by the Polycomb complex. The project’s objectives were to characterize the dynamics of chromatin regulation in models of proliferation and differentiation, to identify molecular mechanisms involved in chromatin modulation, and explore their potential therapeutic applications in cancer.

Outcomes: From 2014 to 2021 we have published 16 papers covering different aspects of the project. These papers describe novel roles of ncRNAs in epigenetic maintenance and cell cycle regulation, describe novel small RNAs for activating gene expression by modulating epigenetic signals in chromatin, and characterize DNA methylation patterns related to altered gene expression in prostate cancer. The project has also produced software tools for handling and analyzing ChIP-seq and other genomic-oriented data.