NTNU - HUNT - samples
Biological samples from The HUNT Study
Different sorts of biological materials were collected in The HUNT Study.
HUNT1 collected only serum among those with known diabetes.
HUNT2 collected serum and full blood, and DNA was extracted from the last.
HUNT3 and HUNT4 aimed for the collection of different sample types from all participants, and established fixed procedures for sample taking, transport and storage. Part of the samples were analyzed immediately, and the results of these analyses are available from our databank. Some samples were stored for later use, and it is possible to apply for use of these samples in research projects.
In addition to samples, HUNT Research Centre offers the results of analyses from earlier projects - see variables at HUNT Databank.
Also, GWAS analyses were performed for all participants HUNT received blood samples from. More about the GWAS analyses can be found on the website of the K.G. Jebsen Center for Genetic Epidemiology. You can also find information on how to apply for the use of GWAS data, and a number of SNP's with known associations from previous studies
The table below gives an overview of sample types and numbers in The HUNT Study as a whole and how they are stored.
| HUNT1 | HUNT2 | HUNT3 | Young-HUNT3 | HUNT4 | Young-HUNT4 | Liquid Nitrogen | -80 d. Celcius | -20 d. Celcius | Room temperature | |
|---|---|---|---|---|---|---|---|---|---|---|
| Participation (1) | 77721 | 65237 | 50807 | 8200 (2) | 55500 | 8124 | ||||
| DNA | 63000 | 28100 (3) | 18700 (4) | x | x | |||||
| Buffy coat | 48100 | 52800 | x | |||||||
| ACD | 40700 | x | ||||||||
| NaHep | 25400 | x | x | |||||||
| Plasma | 49000 | 53100 | x | x | ||||||
| Serum | 473 | 64500 | 50100 | 54500 | x | x | ||||
| Tempus, RNA | 14800 | x | ||||||||
| Urine (spot urine) | 12000 | 26900 | x | |||||||
| Urine, MA | 5700 | x | ||||||||
| FTA card | 8400 | x | ||||||||
| Feces | 13300 | x | ||||||||
| Saliva | 17100 | x | ||||||||
| Saliva (Oragene DNA) | 7100 | x |
(1) S Krokstad, A Langhammer, K Hveem, TL Holmen, K Midthjell, TR Stene, G Bratberg, J Heggland and J Holmen. Cohort Profile: The HUNT Study, Norway. Int J Epidemiol. 2013 Aug;42(4):968-77. doi: 10.1093/ije/dys095.
(2) Turid Lingaas Holmen, Grete Bratberg, Steinar Krokstad, Arnulf Langhammer, Kristian Hveem,Kristian Midthjell, Jon Heggland, Jostein Holmen. Cohort profile of the Young-HUNT Study, Norway: A population-based study of adolescents. Int J Epidemiol (2014) 43 (2): 536-544.
(3) DNA is available from HUNT3 participants who did not participate in HUNT2. We have buffy coat available from nearly all HUNT3 participants, and can extract DNA on request.
(4) DNA is available from HUNT4 participants who did not participate in HUNT3. We have buffy coat available from nearly all HUNT3 participants, and can extract DNA on request.
Sample numbers as per 01.01.2021