Mini2P - the brain explorer!

Mini2P - the brain explorer!

Mini2P is an open-source miniature 2-photon microscope brain explorer for fast high-resolution calcium imaging in freely-moving mice.


Say hello to Mini2P

Say hello to Mini2P

 


In brief

In brief

Development of a miniature 2-photon miniscope for large-scale calcium imaging in freely moving mice allows stable simultaneous recording of more than a thousand cells across multiple planes of densely active cortical regions in a wide spectrum of behavioral tasks without impediment of the animal's behavior.

Highlights

- We made a light-weight 2-photon miniscope for calcium imaging in freely moving mice

- Stable high-quality imaging was observed during a wide spectrum of behaviours

- Activity can be monitored in volumes of over 1,000 visual or entorhinal-cortex cells

- A custom-designed z-scanning module allows fast imaging across multiple planes

Graphical abstract

Graphical abstract from the paper. Illustration.


The Mini2P assembly tutorial

The Mini2P assembly tutorial


Build your Mini2P

Build your Mini2P

Mini2P is open source and available on GitHub

Mini2P is a complete open-source project, we encourage people to use, test, modify and further develop this toolbox.

Mini2P_toolbox on GitHub includes the codes, softwares, 3D models, protocols, and etc. for building and using Mini2P to do freely-moving recording.


Read the paper in Cell

Read the paper in Cell

Mini2P is created by Weijian Zong et al. at the Moser group at Kavli Institute for Systems Neuroscience in Trondheim:

Zong, et al.,"Large-scale two-photon calcium imaging in freely moving mice" Cell (2022).

Blue

 


Mini2P cell signal

Mini2P signal


Mini2P logo

Mini2P logo


Workshop

Workshop

1st international Mini2P workshop

Dec 2022: We learned to build and use the Mini2P for three days in lovely Trondheim with Weijian Zong and the team at Kavli Institute. The workshop covered all aspects of Mini2P development, assembly, image acquisition, baseplating and use of the miniscope in freely-moving mice.

-> Learn more about the workshop!


Connect with the Mini2P

Connect with the Mini2P


Connect with the Mini2P team

Connect with the Mini2P team

Questions? Suggestions? Found bugs in the codes?

Get in touch with the Mini2P team headquartered at The Kavli Institute at NTNU in Trondheim: Join the Slack-team!

Mini2P-team at Kavli Institute for Systems Neuroscience. Photo and link to  Slack-team.
The Mini2P team, back from the left: Edvard Moser, Martin Pofahl, Torstein Slettmoen, Nienke L. de Jong, Horst A. Obenhaus, May-Britt Moser, Klaus Jenssen. In front, from the left: Hanna Eneqvist, Emile R. Skytøen, Marina R. Jorge, Ruben Vale and Ragnhild I. Jacobsen. Weijian Zong was not present the day the photo was taken.

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